A thorough explanation of one of the UV-visible absorbance detectors, DAD. We will also clearly explain the differences with VWD! Basic knowledge materials are available for free.
DAD, unlike VWD, irradiates the cell with light from the light source without spectrally analyzing it. The light that passes through the cell is spectrally analyzed and detected by a device with an array of photodiodes (photodiode array).
It is possible to record the UV-Vis spectrum of the eluent from the column. Therefore, simultaneous multi-wavelength detection, peak purity testing, and comparison with the UV-Vis spectrum of standard substances can be performed, making the peak identification capability superior to that of VWD.
This document provides a clear summary of the fundamentals of separation in liquid chromatography (LC), detectors, and principles. It is recommended for those who are newly learning HPLC, those who want to review from the basics, and those looking for materials useful for in-house training.
[Contents (partial)]
■ Introduction
■ HPLC Equipment
■ Separation in HPLC
■ Detection
*For more details, please download the PDF or feel free to contact us.